Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al.assess the ability 1100 of low-concentration Matrigel conditions to promote intestinal organoid growth.Their results suggest Chains that a low-viscosity culture system can improve live cell yield compared to the existing dome method, while maintaining similar morphology, and represents a useful approach for high-throughput applications of organoids.